金钱豹猫瘟热疫苗免疫后血清抗体水平的检测

金钱豹经2种猫瘟热灭活疫苗和1种弱毒疫苗免疫接种后,间隔不同的时间采血分离血清,用SPA-ELISA和HI两种方法测定猫瘟热血清抗体效价。结果猫瘟热灭活疫苗和弱毒疫苗均能有效诱导金钱豹产生较高效价的抗体,抗体持续时间相对较长。3种疫苗免疫效果比较显示,美国Fort Dodge猫三联灭活疫苗免疫效果最好。在猫瘟热血清抗体检测中,SPA-ELISA和HI的吻合率达到93%以上。     

秦川牛超数排卵效果的研究

本研究共超排秦川牛31头次，有效超排20头次，有效率为64.5％。头均获胚总数6.45枚，可用胚4.20枚。激素用量为300 mg/头时，头均获胚总数和头均可用胚数显著高于260和340 mg组（P<0.05）。冬春季的头均获胚总数（8.56）和头均可用胚数（6.00）显著高于夏秋季（4.73、2.73）（P<0.05）。对供体牛连续超排处理3次，间隔70 d，对超排效果无显著影响。     

养猪小区粪便资源化处理工艺设计

影响我国养猪小区健康发展的关键问题是养猪小区的环境控制，降水、污水、粪便混合排放，加剧了养猪小区的环境污染，使粪便资源化处理更加困难。根据现代养猪生产工艺流程，利用工程技术手段，设计了一个现代化示范型养猪小区粪便资源化处理工艺，首先解决天然降水与污水混合排放问题，其次实现污水与粪便有效分离，第三步通过充氧发酵、搅拌干燥最终实现养猪小区粪便资源化开发利用，解决捆扰我国养猪小区健康发展的环境控制问题。     

3个黄牛品种的myostatin 5''调控区多态与生长性状的相关分析

用PCR-RFLPs方法对南阳牛、秦川牛、郏县红牛3个品种共411个个体的myostatin 5'调控区的单核苷酸多态性(SNPs)进行分析.结果表明,3个品种在myostatin 5'调控区T→A突变以等位基因T为主,仅在郏县红牛中发现1个AA型纯合体,郏县红牛品种显著偏离Hardy-Weinberg平衡状态(P＜0.05),其它品种处于Hardy-Weinberg平衡状态(P＞0.05).对黄牛myostatin 5'调控区T→A突变与南阳牛、秦川牛和郏县红牛生长性状进行相关分析,结果显示,TT型南阳牛6月龄的胸围和胸围指数显著低于TA型个体,TT型南阳牛18月龄的体高显著高于TA型个体(P＜0.05),但基因型对秦川牛和郏县红牛生长性状的效应不显著(P＞0.05).提示在南阳牛的育种实践中应该综合考虑南阳牛生长阶段和所要选择的性状.     

动物消化道乳酸菌粘附机制及影响因素研究进展

消化道益生乳酸菌群对动物健康起着非常重要的作用。乳酸菌通过粘附作用与宿主消化道上皮细胞紧密结合，形成生理性菌膜。乳酸菌黏附上皮细胞的机理有多种学说，主要包括乳酸菌粘附的特异性与非特异性、乳酸菌粘附的表面分子与肠道粘膜上皮细胞粘附受体学说等。影响乳酸菌与肠道上皮细胞粘附和定植的因素主要包括PH值、Ca^2＋浓度和温度。     

亚麻根系活力与主要农艺性状的相关性研究

对亚麻根系活力与地上部主要农艺性状进行了研究,结果表明:亚麻根系的还原强度以枞形期至现蕾期最高,以后逐渐递减,其中上段根递减最快,其次为中段根,下段根比较平稳。在枞形期和快速生长期,上段和中段根的还原强度分别比下段根高出37.78%~84.6%和20.40%~45.39%,进入成铃期,三段根的还原强度几乎无差异。还原强度与单株果数、单株粒重、单株花朵数呈高度正相关,与结实率呈负相关,根系干重的增长符合Log istic生长方程,根系干重与平均增长速率、渐增期增长速率、快增期增长速率呈极显著正相关,与增长期长短关系不显著,根系干重与单株果实数,单株粒重,单株花朵数呈极显著正相关,与结实率呈显著负相关。     

西吉县小麦丰产抗锈病品种综合分析选优

2003~2005年进行了冬春小麦42个水旱地品种的比较试验,运用灰色关联综合评价方法,筛选出西吉县丰产抗条锈病品种:冬小麦水地品种静冬9717、静冬941、静冬9718,旱地品种8802、陇原935、陇原992综合性状较好且抗条锈病;春小麦水地品种宁春4号、802-15、定丰806;旱地品种93-163-6、陇春8139-2综合性状较优,除宁春4号较易感条锈病外,其它品种均抗锈病。抗病品种比感病品种提高产量15.40%~61.12%。建议今后在西吉县大面积推广种植这些品种时,水浇地要特别注意宁春4号品种的条锈病预测及防治工作。     

河西走廊水资源特征及其循环转化规律

河西走廊水资源主要以雪冰水资源、地表水资源与地下水资源的形式存在,分布于石羊河、黑河、疏勒河3大相对独立的流域水系.南部祁连山区发育大小河流共计57条,多年平均出山径流量71.29×108 m3,总体而言,近50 a出山径流变化比较稳定.走廊平原主要由8个大型的构造地貌盆地组成,盆地地下水主要接受出山河水及引灌河水的入渗补给,是地表水资源的重复表现形式,多年平均地下水补给量为42.42×108 m3.近50 a来,地下水补给量减少了17.19×108 m3,由此引起泉水资源23 a来衰减了24.6%.受构造地貌的制约,自南部山区至北部盆地,地下水与河水之间经过5个不同地带有规律的、大数量的、重复的转化过程,形成完整统一的"山区地下水-地表水-南盆地地下水-地表水(泉水)-北盆地地下水"水资源循环系统.     

Isolation,Identification and Biological Characteristics of Staphylococcus chromogenes from Tibetan Pigs

In order to understand the infection of Staphylococcus disease in healthy Tibetan pigs in Linzhi city,Tibet.232 samples were collected from the slaughterhouse in Bahe town,Gongbujiangda county,Linzhi city,Tibet,the teaching practice ranch at the Tibet Agriculture and Animal Husbandry University in Bayi district,Linzhi city,and the slaughterhouse in Bayi district,Linzhi city.These samples were isolated,cultured and identified,and one isolated strain was systematically studied for biological characteristics.Epidemiological survey results showed that 73 of 232 samples were positive for Staphylococcus,the positive rate was 31.47%,16 positive samples were detected in the slaughterhouse of Bahe town,Gongbujiangda county,Linzhi,Tibet,with a detection rate of 23.89%(16/67),43 positive samples were detected in the pastures of the teaching practice ranch at the Tibet Agriculture and Animal Husbandry University in Bayi district,Linzhi city,with a detection rate of 34.40% (43/125),14 positive samples were detected in the slanghterhouse in Bayi district,Linzhi city,with a detection rate of 35.00% (14/40).Through PCR identification,gene sequencing and biochemical identification,5 strains of Staphylococcus chromogenes were isolated,and one of them (Sa LZ1) was studied systematically.The biological characteristics of the isolated strains showed that the Sa LZ1 strain formed round white convex colonies on the agar medium,with regular edges,smooth surfaces,and opaque white colonies.The isolates were Gram-positive cocci bacteria.PCR sequencing results showed that the isolate had the highest homology with Staphylococcus chromogenes (GenBank accession No.:MG576253.1),positive for serum inulin,manicol,glucose,saccharose,lactose,negative for arabinose,sodium hippurate,raffinose,xylose,urea,etc,consistent with the biochemical characteristics of Staphylococcus chromogenes.The logarithmic growth period of isolated bacteria was 5 to 10 h.The pathogenicity test of mice showed that the mortality rate of mice reached 100% when the concentration of Sa LZ1 strain reached 3.5×10⁹ CFU/mL.And the results of pathological section showed that Sa LZ1 strain could cause the visceral tissue of mice to develop pathological changes.The above results provided a theoretical basis for the prevention and control of Staphylococcus suis in Linzhi,Tibet.     

干奶期不同能量水平对奶牛血中神经肽 Y和生长激素浓度的影响

将健康围产期奶牛30头随机分为3组．预产前28d按奶牛营养需要分别饲喂100％、120％、80％能量日粮，产后各组奶牛均饲喂标准的产奶日粮，至产后56d结束，观测干奶期不同能量摄入水平对围产期健康奶牛血中神经肽Y和生长激素浓度的影响。结果表明，干奶期低能量日粮饲喂，奶牛血中神经肽和生长激素浓度均高于其他2组，从产前14d至产后28d组间差异显著（P〈0.01；P〈0.05），提示低能量饲料饲喂干奶期奶牛，产后能量负平衡得到缓解。